U1 snRNP68/70 (Human U1 Small Nuclear Ribonucleoprotein particle 68/70)

U1 snRNP68/70 (Human U1 Small Nuclear Ribonucleoprotein particle 68/70)

SKU: 41500

★ Download Datasheet PDF ★

 

 

Origin:

Recombinant

Cat. No.:

41500

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodopterafrugiperda Sf9

Purity:

>90%

Other names:

SnRNP70,RNP68/70, RNP68, RNP70

Species:

Human

 

  • INTRODUCTION

    Small nuclear ribonucleoprotein complexes are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B', D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients.

  • IMMUNOLOGICAL FUNCTION

    As an autoantigen, RNP68/70 binds with IgG-type human auto-antibodies.

  • AMINO ACID SEQUENCE

    MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQGATQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRHSGRDDTSRYDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRRSRERSKDKDRDRKRRSSRSRERARRERERKEELRGGGGDMAEPSEAGDAPPDDGPPGELGPDGPDGPEEKGRDRDRERRRSHRSERERRRDRDRDRDRDREHKRGERGSERGRDEARGGGGGQDNGLEGLGNDSRDMYMESEGGDGYLAPENGYLMEAAPE

  • APPLICATIONS

    Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

  • FORMULATION

    Liquid in 8M Urea buffer(pH 8.0) with protease inhibitor.

  • DESCRIPTION

    Expressed in E.coli with a total 480 AA. Mw: 56.5 kDa (calculated).

    N-terminal 6xHis-tag and TEV cleavage site, 44 extra AA (highlighted).

    Recombinant antigen for research use or manufacturing only.

  • STORAGE

    Store at –80°C.  Avoid repeated freezing/thawing cycles.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

$340.00Price

For bulk order (QTY: 5 or more), please contact us for quote

Featured Products