U1 snRNP68/70 (Human U1 Small Nuclear Ribonucleoprotein particle 68/70)
SnRNP70,RNP68/70, RNP68, RNP70
Small nuclear ribonucleoprotein complexes are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B', D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients.
As an autoantigen, RNP68/70 binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Liquid in 8M Urea buffer with protease inhibitor.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Expressed in E.coli with a total 480 AA. Mw: 56.5 kDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Refer to picture