• Human U1 Small Nuclear Ribonucleoprotein particle 68/70 (U1-snRNP68/70)

     

     

    Origin:

    Recombinant

    Cat. No.:

    41500

    Concentration:

    2.44mg/mL

    Size:

    0.1 mg

    Source:

    Spodopterafrugiperda Sf9

    Purity:

    >90%

    Other Names:

    SNRNP70

    Species:

    Human

    • INTRODUCTION

      Small nuclear ribonucleoprotein complexes are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B', D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients.

    • DATA SHEET

    • IMMUNOLOGICAL FUNCTION

      As an autoantigen, RNP68/70 binds with IgG-type human auto-antibodies.

    • AMINO ACID SEQUENCE

      MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQGATQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRHSGRDDTSRYDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRRSRERSKDKDRDRKRRSSRSRERARRERERKEELRGGGGDMAEPSEAGDAPPDDGPPGELGPDGPDGPEEKGRDRDRERRRSHRSERERRRDRDRDRDRDREHKRGERGSERGRDEARGGGGGQDNGLEGLGNDSRDMYMESEGGDGYLAPENGYLMEAAPE

    • APPLICATIONS

      Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

    • FORMULATION

      Liquid at 2.44 mg/mL in 8M Urea buffer with protease inhibitor.

    • RECONSTITUTION

      Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

       

    • DESCRIPTION

      Expressed in E.coli with total 480 AA. Mw:56.5 kDa(calculated).

      N-terminal 6xHis-tag and TEV cleavage site, 44 extra AA (highlighted).

      Recombinant antigen for research use or manufacturing only.

    • STORAGE

      Store at –80°C.  Avoid repeated freezing/thawing cycles.

    • QUALITY CONTROL TEST

      BCA to determine quantity of the protein.

      SDS PAGE to determine purity of the protein.

    • SDS-PAGE Gel

      Refer to picture 

    $998.00Price
    Quantity

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