mATX/mENPP2 (Mouse Autotaxin)
  • mATX/mENPP2 (Mouse Autotaxin)

    SKU: 42771

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    Origin:

    Recombinant

    Cat. No.:

    42771

    Tag:

    C-terminal 6xHis

    Size:

    0.1 mg

    Source:

    HEK293

    Purity:

    >90%

    Other Names:

    ATX, ENPP2

    Species:

    Mouse

    • INTRODUCTION

      Autotaxin (ATX, ENPP2) is a secreted glycoprotein with phosphodiesterase (PDE) activity. It is one of the member in the nucleotide pyrophosphatase/phosphodiesterase family (NPPs) family. ATX has lysophospholipase D activity that converts lysophosphatidylcholine into LPA, and it was originally identified as a tumor cell-motility-stimulating factor. LPA, which specifically binds to G protein-coupled receptors (GPCR), plays a wide range of biological activities, including cell hyperproliferation, which may contribute to oncogenesis and metastasis. ATX has been to be overexpressed in many tumor cells, and thus plays an important role in tumor development and metastasis. In addition, ATX also plays an important role in nervous and immune systems.

    • DATA SHEET

    • DESCRIPTION

      Expressed in HEK293 cells with total 871 AA. Mw: 100.1 KDa (calculated).

      C-terminal 6xHis-tag, 9 extra AA (highlighted).

      Recombinant protein for research use or manufacturing only.

    • AMINO ACID SEQUENCE

      MARQGCFGSYQVISLFTFAIGVNLCLGFTASRIKRAEWDEGPPTVLSDSPWTNTSGSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYESEISYYHHHHHH

    • STORAGE

      Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

    • RECONSTITUTION

      Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

       

    • QUALITY CONTROL TEST

      BCA to determine quantity of the protein.

      SDS PAGE to determine purity of the protein.

    • FORMULATION

      Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

    • APPLICATION

      ELISA and Western Blotting.

    • SDS-PAGE Gel

      Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

    • ENDOTOXIN LEVEL

      <0.01 EU per 1 μg of the protein by the LAL method.

    • BIOACTIVITY TEST

      Measured by enzymatic ability to cleave Bis (p- Nitrophenyl) Phosphate (BPNPP).

      The specific activity of mouse ATX is > 5,000 pmol/min/μg

      * Specific Activity (pmol/min/µg) = Adjusted Vmax* (OD/min) x Conversion Factor (pmol/OD)/Amount of Enzyme (µg)

    $260.00Price

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