Mouse GDF-15 ELISA kit

Mouse GDF-15 ELISA kit

SKU: 32980

 

★ Download Datasheet PDF ★

Download MSDS

 

Assay range :  3.9 - 250 pg/ml

Size:                  96T/Kit

 

  • INTRODUCTION

    Growth differentiation factor 15 (GDF-15) is a member of the transforming growth factor β cytokine superfamily, the protein is secreted as a 25 kDa disulfide-linked dimer. Circulating GDF-15 levels are associated with cancers, cardiovascular and kidney diseases. GDF-15 regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

  • PRINCIPLE OF THE ASSAY

    This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for mouse GDF-15. Standards and samples are pipetted into the wells and any mouse GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse GDF-15 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    A. Typical representation of standard curve

    The following standard curve is provided for demonstration only.  A standard curve should be generated for each set of sample assay. 

    Mouse GDF-15 (pg/ml)

    Absorbance

    (450 nm)

    Blanked Absorbance

    0

    0.091

    0

    3.9

    0.126

    0.035

    7.8

    0.164

    0.073

    15.6

    0.246

    0.155

    31.2

    0.404

    0.313

    62.5

    0.692

    0.601

    125

    1.259

    1.168

    250

    2.216

    2.12

     

    B. Sensitivity:

     The lowest level of mouse GDF-15 that can be detected by this assay is 3.9 pg/ml.

     

    C. Precision:

    Intra-assay Precision (Precision within an assay) < 2.9%.

    Inter-assay Precision (Precision between assays) < 6.1%.

     

$380.00Price

©2019 by ImmunoDiagnostics Limited.