Mouse GDF-15 ELISA Kit
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Assay range: 3.9 - 250 pg/mL
Kit Size: 96 wells/kit
Other Names: MIC-1, PTGF-b, PDF, PLAB, NAG-1, PL741
INTRODUCTION
Growth differentiation factor 15 (GDF-15) is a member of the transforming growth factor β cytokine superfamily, the protein is secreted as a 25 kDa disulfide-linked dimer. Circulating GDF-15 levels are associated with cancers, cardiovascular and kidney diseases. GDF-15 regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
PRINCIPLE OF THE ASSAY
This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for mouse GDF-15. Standards and samples are pipetted into the wells and any mouse GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse GDF-15 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse GDF-15 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse GDF-15, the unknown sample concentration can be interpolated from a reference curve included in each assay.
ASSAY PERFORMANCE
A. Typical representation of standard curve
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Mouse GDF-15 (pg/mL)
Absorbance
(450 nm)
Blanked Absorbance
0
0.091
0
3.9
0.126
0.035
7.8
0.164
0.073
15.6
0.246
0.155
31.2
0.404
0.313
62.5
0.692
0.601
125
1.259
1.168
250
2.216
2.125
B. Sensitivity
The lowest level of mouse GDF-15 that can be detected by this assay is 3.9 pg/mL.
C. Precision
Intra-assay Precision (Precision within an assay) C.V. <2.9%.
Inter-assay Precision (Precision between assays) C.V. <6.1%.
PUBLICATIONS CITING THIS PRODUCT
- Guo Q, Xu Z, Zhou D, Fu T, Wang W, Sun W, Xiao L, Liu L, Ding C, Yin Y, Zhou Z, Sun Z, Zhu Y, Zhou W, Jia Y, Xue J, Chen Y, Chen XW, Piao HL, Lu B, Gan Z. Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4. Sci Adv. 2022 Jul 29;8(30):eabo0340.