Mouse Recombinant Leptin protein
Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Mouse Leptin shares approximately 84% sequence identity with the human protein. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature.
Recombinant Mouse Leptin without Signal peptide (AA22-167), total 147 amino acids (AA). N-terminal His-tag removed. Mw: 16.1 kDa (calculated). 1 extra AA left (In bold).
AMINO ACID SEQUENCE
Store at –20°C. Avoid repeated freezing/thawing cycles.
Lyophilized in 1 mg/mL in PBS.
Cell culture, animal studies, ELISA and Western blotting.
Add sterile deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.