The healthy non-immunized population of mouse were used to collect serum. IgG was prepared by ammonium sulfate, and column chromatography. The purified mouse IgG was judged to be >95% pure by SDS-Page.

Protein A affinity purification

The control mouse IgG is provided as liquid (1 mg/mL+0.05% sodium azide as preservative) in phosphate- buffered saline (PBS, pH 7.4). Store at 4°C for less than two weeks. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles. Do not store diluted solutions. Sodium azide, an inhibitor of peroxidase, must be avoided if used with antibody-HRP conjugates.

1. Omar BA, Andersen B, Hald J, Raun K, Nishimura E, Ahrén B. Fibroblast growth factor 21 (FGF21) and glucagon-like peptide 1 contribute to diabetes resistance in glucagon receptor-deficient mice. Diabetes. 2014 Jan;63(1):101-10.