Mouse Fibroblast Growth Factor 21 (FGF-21), His-tag
FGF-21, a polypeptide with 210 amino acid residues produced mostly from the liver tissue. Mouse FGF- 21 shares 75% identity as human FGF-21. Recent animal studies indicate it possesses potent beneﬁcial effects on glucose and lipid metabolism and insulin sensitivity. Increasing data shows FGF-21 can significantly stimulate glucose uptake in mature adipocytes. And the lowered LDL-cholesterol and increased HDL-cholesterol can also be observed. FGF-21 exerts its bioactivity through interaction with membrane bound FGF receptors (FGFRs) which requires β-Klotho as a co-factor to bind and activate FGFR signaling. The activation of FGF-21 can induce the stimulation of diverse downstream pathways medicated by MAPK,FRS-2, SHP-2, PI3K, raf, stat and other signaling molecules. In sum, FGF-21 induces a variety of significant beneficial metabolic changes without apparent adverse effects which makes this factor a hot candidate to treat some metabolic diseases.
Total: 207 AA. Mw: 23kDa (calculated).
N-terminal His- tag and TEV cleavage site, 25 extra AA (highlighted).
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.Avoid repeated freezing /thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and letthe lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized in 1 mg/mL in PBS.
ELISA and Western blotting.
 Nishimura T, Nakatake Y, Konishi M, Itoh N: Identiﬁcation of a novel FGF, FGF-21, preferentially expressed in the liver. Biochim Biophys Acta1492:203–206, 2000
 Kharitonenkov A et al. FGF-21 as a novel metabolic regulator. J Clin Invest 115:1627–1635, 2005
Alexei Kharitonenkov et al. The Metabolic State of Diabetic Monkeys Is Regulated by Fibroblast Growth Factor-21.Endocrinology .148(2):774–781. 2007
 Hiroshi Kurosu et al. Tissue-specific Expression of βKlotho and Fibroblast Growth Factor (FGF) Receptor Isoforms Determines Metabolic Activity of FGF19 and FGF21. J Biol Chem. 282(37): 26687–26695. 2007
 Ogawas Y et al. BetaKlotho is required for metabolic activity of fibroblast growth factor 21 Proc Natl Acad Sci USA 104: 7432-7437, 2007.
Steven L. PELECH et al. Fibroblast growth factor treatment of Swiss 3T3 cells activates a
subunit S6 kinase that phosphorylates a synthetic peptide substrate. Proc. Natl. Acad. Sci. USA
Vol. 83, pp. 5968-5972, August 1986
Rosa Carballada et al. Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathway during Xenopus mesoderm induction. Development 128, 35-44 (2001)
Dayanand D. Deo et al. Phosphorylation of STAT-3 in Response to Basic Fibroblast Growth
Factor Occurs through a Mechanism Involving Platelet-activating Factor, JAK-2, and Src in Human Umbilical Vein Endothelial Cells. The journal of molecular chemistry. Vol. 277, No. 24, Issue of June 14, pp. 21237–21245, 2002
Maria I. Kontaridis et al. Role of SHP-2 in Fibroblast Growth Factor Receptor-Mediated
Suppression of Myogenesis in C2C12 Myoblasts. Molecular and cellular biology, Vol. 22, No. 11, p. 3875–3891. 2002
 Dostálová I, Haluzíková D, Haluzík M.Fibroblast Growth Factor 21: A Novel Metabolic Regulator With Potential Therapeutic Properties in Obesity/Type 2 Diabetes Mellitus. Physiol. Res. 58: 1-7, 2009