Mouse Major Urinary Protein-1 (MUP-1)
Mup7, Up-1, Ltn-1, Mup-1, Mup- a, Mup10, Lvtn-1
Major urinary protein 1 (Mup1), also known as Mup7, Up-1, Ltn-1, Mup-1, Mup-a, Mup10 and Lvtn-1, is a low molecular weight secreted protein produced predominantly from the liver. Structurally it belongs to the lipocalin family, which carries small hydrophobic ligands such as pheromones. Mup1 is an important player in regulating energy expenditure and metabolism in mice, and its deficiency might contribute to the metabolic dysregulation in obese/diabetic mice.
Total 190 AA. Mw: 22 KDa (calculated). N-terminal His-tag and TEV cleavage site, with 28 extra AA (highlighted).
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized in PBS.
ELISA and Western Blotting.
<0.01EU per 1µg of protein by LAL method.