Serum Amyloid A Turbidimetric Immunoassay Reagent Kit
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Serum Amyloid A (SAA) is a family of apolipoproteins associated with high-density lipoprotein in plasma. There are 4 different isoforms of SAA, named SAA1 to SAA4, encoded by 4 distinct genes. SAA are produced predominantly by the liver. The main functions of SAA include the transport of cholesterol to the liver for secretion into the bile, the recruitment of immune cells to inflammatory sites, and the induction of enzymes that degrade extracellular matrix.
SAA is a kind of acute phase marker that responds to inflammatory stimuli. Levels of acute-phase SAA increase within hours after inflammatory stimulus, even for trivial inflammatory stimuli. The result for an SAA test is positive when the SAA level exceeds 10 mg/L.
The sensitivity is defined as the lower limit of detection and is estimated as the mean of the blank sample plus three times the SD obtained from the blank sample. The sensitivity of SAA assay is 0.123mg/L.
The precision of the SAA assay is < 10% CV. Two samples consisting of serum based panels were assayed 20 times separately.
The SAA assay is linear between 1 mg/L to 400 mg/L.
No interference was detected with hemoglobin up to 5 g/L, conjugated bilirubin up to 300 mg/L, free bilirubin up to 300 mg/L, and up to 5g/L lipid emulsion.