Serum Amyloid A ( SAA ) Turbidimetric Immunoassay Kit
Serum Amyloid A (SAA) is a family of apolipoproteins associated with high-density lipoprotein in plasma. There are 4 different isoforms of SAA, named SAA1 to SAA4, encoded by 4 distinct genes. SAA are produced predominantly by the liver. The main functions of SAA include the transport of cholesterol to the liver for secretion into the bile, the recruitment of immune cells to inflammatory sites, and the induction of enzymes that degrade extracellular matrix.
SAA is a kind of acute phase marker that responds to inflammatory stimuli. Levels of acute-phase SAA increase within hours after inflammatory stimulus, even for trivial inflammatory stimuli. The result for an SAA test is positive when the SAA level exceeds 10 mg/L.
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of SAA in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with SAA antibodies, is added into the cuvette and mixed. The presence of SAA in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of SAA in unknown samples can be interpolated from a reference curve using the standards provided.
PERFORMANCE OF THE KIT
TYPICAL STANDARD CURVE
Absorbance (570 nm)
Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:
1. Dispense 150µl of R1 into a clean cuvette
2. Add 1.5µl of serum or plasma samples, SAA calibrators or controls and incubate at 37˚C for 5 minutes
3. Further add 50µl of R2
4. Read change of absorbance at Main Wavelength 570 nm for 8 minutes after the addition of R2
5. Calculate the concentration of SAA in unknown sample by interpolation from a reference curve using the standards provided
R1: 15ml, R2: 5ml
R1: 30ml, R2: 10ml
R1: 60ml, R2: 20ml
R1: 150ml, R2: 50ml
R1: 300ml, R2: 100ml
For bulk order, please enquire