Anti-human STAP1 Antibody, Rabbit pAb

Signal-transducing adaptor protein 1 (STAP1; also known as BCR downstream­signaling protein 1, BRDG1) is a 37 kDa adaptor protein which is involved in B cell antigen receptor signaling. This protein is a substrate of tyrosine-protein kinase Tec, and its interaction with tyrosine-protein kinase Tec is phosphorylation-dependent. Human STAP1 is 295 amino acids in length and contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. Human STAP1 shares 82% amino acid identity with mouse STAP1.

E. coli­ derived recombinant human STAP1

Accession # Q9ULZ2

Antigen-affinity purified Polyclonal rabbit IgG

Rabbit specific IgG was purified by autotaxin affinity chromatography

Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Affinity chromatography on a column with immobilized protein G.

Human. Not yet tested in other species

Solution in PBS.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Detection of Human BRDG1 by Western Blot. 

Western blot shows B cell fractionation of splenic B cells either C57BL/6 STAP1 KO or WT mice. PVDF membrane was probed with 8 µg/mL of Human STAP1 Antigen Affinitypurified Polyclonal Antibody (Catalog # 11A121) followed by HRP conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for STAP1 at approximately 37 kDa (as shown in picture). 

Western blot – recommended concentration 1~5 ug/ml