Adiponectin Antibody
Size: 5 mg Host: Rabbit
INTRODUCTION
Adiponectin, also termed gelatin-binding protein-28 (GBP28), AdipoQ, ACP30 (Acrp30), or apM, is a major adipocyte-secreted adipokine which abundantly present in the circulation as three distinct oligomeric complexes: LMW(67kDa), MMW(167kDa) and HMW(300kDa) adiponectin. Its levels are decreased in insulin resistant, diabetes and cardiovascular disease. Conversely, elevation of circulating adiponectin concentrations can alleviate various vascular dysfunctions in animal models, suggesting this adipokine exerts vasculoprotective effects. In addition, adiponectin may also be of importance in the development and progression of several malignancies.
REFERANCE
[1] Xu A, et al. (2005) Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes. J. Biol. Chem. 280, 18073–18080
[2] Xu A, et al. (2008) Selective Elevation of Adiponectin Production by the Natural Compounds Derived from a Medicinal Herb Alleviates Insulin Resistance and Glucose Intolerance in Obese Mice. Endocrinology. [Epub ahead of print]
[3] Xu A, et al. (2004) Adiponectin ameliorates dyslipidemia induced by the human immunodeficiency virus protease inhibitor ritonavir in mice. Endocrinology. 145(2):487-94
[4] Wang Y, et al. (2008) Post-translational modifications of adiponectin: mechanisms and functional implications. Biochem J. 409(3):623-33
ISOTYPE/ PREPARATION
Rabbit crude IgG was purified by protein-G column
IMMUNOGEN
Recombinant full-length human adiponectin expressed in mammalian cells
SPECIFICITY
The antibody detects three types of circular human adiponectin and monomer (30kDa) adiponectin
FORMULATION
Solution in PBS. Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
APPLICATION/ USAGE
Western blot - This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human adiponectin.
ELISA - This antibody can be used at 0.5 - 1.0 μg/mL with the appropriate secondary reagents to detect human Adiponectin.
QUALITY CONTROL TEST
BCA to determine quantity of the antibody.
DATA SHEET