Affinity-purified Polyclonal Antibody against SARS-CoV-2 Spike Protein
  • Affinity-purified Polyclonal Antibody against SARS-CoV-2 Spike Protein

    SKU: 41A251

     

     

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    Cat. No.: 41A251                                 Size:  100µg                               Host: Rabbit

    • INTRODUCTION

      The SARS-CoV-2 glycosylated spike (S) protein highly exposed on the viral surface is a major determinant for virus binding and invasion into hose cells, which is a main target for neutralization antibody. The receptor-binding domain (RBD) in SARS-CoV-2 S protein is responsible for binding to human and bat angiotensin-converting enzyme 2 (ACE2) receptors.

    • IMMUNOGEN

      Mammalian cell-expressed recombinant SARS-CoV-2 S1RBD.

    • PURIFICATION

      Affinity purification with recombinant SARS-CoV-2 S1RBD as an affinity ligand.

    • ISOTYPE

      Mainly IgG

    • FORMULATION AND STORAGE

      100 μg in PBS with 0.02% azide. Store at –20°C. For long-term storage, aliquot and freeze at -80°C. Avoid repeated freeze/defrost cycles. Spin the tube briefly before opening the tube.

    • APPLICATION/USAGE

      ELISA: This antibody can be used at 1-3 μg/ml with appropriate secondary detection antibody to detect SARS-CoV-2 S or S1RBD.

      Western blot: This antibody can be used at 1-2 μg/ml followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody to detect SARS-CoV-2 S protein orS1RBD.

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