Affinity-purified Polyclonal Antibody against SARS-CoV-2 Spike Protein
The SARS-CoV-2 glycosylated spike (S) protein highly exposed on the viral surface is a major determinant for virus binding and invasion into host cells, which is a main target for neutralization antibody. The receptor-binding domain (RBD) in SARS-CoV-2 S protein is responsible for binding to human and bat angiotensin-converting enzyme 2 (ACE2) receptors.
Mammalian cell-expressed recombinant SARS-CoV-2 S1RBD.
Affinity purification with recombinant SARS-CoV-2 S1RBD as an affinity ligand.
FORMULATION AND STORAGE
100 μg in PBS with 0.02% azide. Store at –20°C. For long-term storage, aliquot and freeze at -80°C. Avoid repeated freeze/defrost cycles. Spin the tube briefly before opening the tube.
ELISA: This antibody can be used at 1-3 μg/ml with appropriate secondary detection antibody to detect SARS-CoV-2 S or S1RBD.
Western blot: This antibody can be used at 1-2 μg/ml followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody to detect SARS-CoV-2 S protein or S1RBD.