Human Angiotensin-Converting Enzyme 2 with Fc Tag (ACE2)
Origin: | Recombinant | Cat. No.: | 41A266 |
Tag: | Fc at C-terminus | Size: | 0.1 mg |
Source: | CHO | Purity: | >95% as determined by SDS-PAGE |
Endotoxin: | <5 EU/mg, determined by the LAL method. | Species: | Human |
INTRODUCTION
Angiotensin-converting enzyme 2 (ACE-2) consists of 805 amino acids, including an N-terminal signal peptide a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail.
ACE2 can serve as the receptor for SARS-CoV and SARS-CoV-2 viruses. Binding of the S1 subunit of the SARS-CoV-2 Spike protein to the ACE2 triggers viral entry into the host cell. Therefore, the interaction between SARS-CoV-2 and ACE2 has become an area of interest to develop therapeutic treatments to fight against COVID-19.
DESCRIPTION
A DNA sequence encoding the signal peptide (Met1-Ala17) and extracellular domain of human ACE2 (Gln18-Ser740) (Uniprot No. Q9BYF1) was expressed with a human IgG1 Fc tag at the C-terminus linked by a flexible linker (GGGGS)2. The predicted molecular mass of ACE2 is 112 kDa for monomer and 224 kDa for dimer. The concentration of protein was determined by BCA.
AMINO ACIDS SEQUENCE
MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSGGGGSGGGGSEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
STORAGE
Lyophilized protein can be stored at 2℃ to 8℃ for short-term, and at -20℃ to -80℃ for long term store.It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Immunodot analysis to determine functionality of protein.
FORMULATION
Lyophilized from sterile PBS, pH 7.4
RECONSTITUTION
For reconstitution, add 100 μl of deionized water, mix gently and incubate the reconstituted product for 10 minutes at room temperature prior to use. Reconstituted protein should be kept at -20℃ to -80℃.