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Angiotensin-converting enzyme 2 (ACE2), human, Recombinant (Fc Tag)

Angiotensin-converting enzyme 2 (ACE2), human, Recombinant (Fc Tag)

SKU: 41A266

★ Download Datasheet PDF ★

 

 

Origin:

Recombinant

 

Source:

CHO

  

Species:

Human

 

 

Tag:

Fc at C-terminus

 

Cat No.

41A266

  

Size:

0.1 mg

  

Purity:

>95% as determined by SDS-PAGE

  

Endotoxin:  <5 EU/mg, determined by the LAL method

  • INTRODUCTION

    Angiotensin-converting enzyme 2 (ACE-2) consists of 805 amino acids, including an N-terminal signal peptide a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail.

    ACE2 can serve as the receptor for SARS-CoV and SARS-CoV-2 viruses. Binding of the S1 subunit of the SARS-CoV-2 Spike protein to the ACE2 triggers viral entry into the host cell. Therefore, the interaction between SARS-CoV-2 and ACE2 has become an area of interest to develop therapeutic treatments to fight against COVID-19.

  • DESCRIPTION

    A DNA sequence encoding the signal peptide (Met1-Ala17) and extracellular domain of human ACE2 (Gln18-Ser740) (Uniprot No. Q9BYF1) was expressed with a human IgG1 Fc tag at the C-terminus linked by a flexible linker (GGGGS)2. The predicted molecular mass of ACE2 is 112 kDa for monomer and 224 kDa for dimer. The concentration of protein was determined by BCA.

  • AMINO ACIDS SEQUENCE

    MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSGGGGSGGGGSEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

  • STORAGE

    Lyophilized protein can be stored at 2℃ to 8℃ for short-term, and at -20℃ to -80℃ for long term store.It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

    Immunodot analysis to determine functionality of protein.

  • FORMULATION

    Lyophilized from sterile PBS, pH 7.4

  • RECONSTITUTION

    For reconstitution, add 100 μl of deionized water, mix gently and incubate the reconstituted product for 10 minutes at room temperature prior to use. Reconstituted protein should be kept at -20℃ to -80℃.

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