Jo-1 (Histidy-tRNA Synthetase)

SKU: 41470

Origin:

Recombinant

Cat. No.:

41470

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodoptera frugiperda Sf9

Purity:

>95%

Other Names:

HARS

Species:

Human

  • INTRODUCTION

    During mRNA translation at ribosomes, specific amino-acyl-tRNA synthetases are required for the

    covalently attachment between tRNAs and different amino acids.

    Histidyl-tRNA synthetase (Jo-1) is specific for the amino acid histidine. Jo-1 is a frequent target of

    autoantibodies in the human autoimmune disease polymyositis and/or dermatomyositis.

  • DATA SHEET

  • IMMUNOLOGICAL FUNCTION

    As an autoantigen, Jo-1 binds with IgG-type human auto-antibodies.

  • AMINO ACID SEQUENCE

    MSYYHHHHHHDYDIPTTENLYFQGAAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

  • APPLICATIONS

    Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

  • FORMULATION

    Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300mM NaCl, 10% glycerol, PH8.0).

  • RECONSTITUTION

    Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilizedpellet dissolve completely.

  • DESCRIPTION

    Expressed in insect Sf9 cells with total 533 AA. Mw: 60.4 KDa (calculated).

    N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

    Recombinant antigen for research use or manufacturing only.

  • STORAGE

    Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeatedfreezing /thawing cycles.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • SDS-PAGE Gel

    Refer to picture 

$998.00Price
Quantity

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