Anti-human CRP Antibody, Mouse mAb, clone 7E12
Monoclonal Antibody against Human CRP
Size: 0.1 mg
C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.1 It has long been recognized that CRP is closely related to immunology, inflammation and host defense; as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.2-4 Normally CRP is presenting only in a trace amount in circulation (<1 g/ml)5-6 but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 g/ml, 1-3 g/ml and >3 g/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.7 Therefore, blood CRP level has become a promising measure of CVD risk.8-9
E.coli-derived recombinant human C-Reactive Protein (CRP)
ISOTYPE/ LIGHT CHAIN TYPE
Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
This antibody can be used as a detection antibody in a human CRP ELISA in combination with monoclonal anti-human CRP antibody (Cat. No.: 21120)
Affinity chromatography on a column with immobilized protein G.
1. Thompson D., Pepys M.B. and Wood S.P. (1999) Structure, 7, 169-177.
2. Festa A, D’Agostino R. Jr., Tracy R.P. and Haffner S.M. (2002) Diabetes, 51, 1131-1137.
3. Verma S. and Yeh E.T. (2003) Am J Physiol, 285, R1253-R1258.
4. Jialal I., Devaraj S. and Venugopal S.K. (2004) Hypertension, 44, 6-11.
5. Kindmark C.O. (1972) Scand J Clin Lab Invest, 29, 407-411.