Anti-human CRP Antibody, Rabbit pAb
C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.1 It has long been recognized that CRP is closely related to immunology, inflammation and host defence, as a result it has been used as an inflammatory marker. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.2-4 Normally CRP is presenting only in a trace amount in circulation (<1 µg/ml)5- 6 but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 µg/ml, 1-3 µg/ml and >3 µg/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.7 Therefore, blood CRP level has become a promising measure of CVD risk.8-9
Recombinant full-length human APPL2 expressed in E. coli cells
The antibody detects human CRP.
Solution in PBS.
Store at –20°C. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
ELISA - This antibody can be used at 0.1 - 0.2 µg/ml with the appropriate secondary reagents to detect human CRP.
1. Thompson D., Pepys M.B. and Wood S.P. (1999) Structure, 7, 169-177.
2. Festa A, D’Agostino R. Jr., Tracy R.P. and Haffner S.M. (2002) Diabetes, 51, 1131-1137.
3. Verma S. and Yeh E.T. (2003) Am J Physiol, 285, R1253-R1258.
4. Jialal I., Devaraj S. and Venugopal S.K. (2004) Hypertension, 44, 6-11.
5. Kindmark C.O. (1972) Scand J Clin Lab Invest, 29, 407-411.
6. Macy E.M., Hayes T.E. and Tracy R.P. (1997) Clin Chem, 43, 52-58.
7. Ridker P.M. (2004) Am Heart Hosp J, 2 (4 Suppl 1), 4-9.
8. Benzaquen l.R., Yu H. and Rifai N. (2002) Crit Rev Clin Lab Sci, 39, 459-497.
9. Pearson T.A. et al., (2003) Circulation, 107, 499-511.
Rabbit IgG was purified by protein-G column. Immunogen: Recombinant Human C- reactive Protein (CRP) expressed in E.coli.