G-CSF, Human, E.coli
Species: | Human |
Expression System: | E.coli |
MW: | 19 kDa (reducing) |
Accession: | P09603-3 |
Cat No.: | UA040043 |
Endotoxin: | ＜1 EU/μg |
Purity: | >95% as determined by SDS-PAGE |
INTRODUCTION
Granulocyte-colony stimulating factor (G-CSF) is a hematopoietic growth factor that stimulates the proliferation and differentiation of neutrophil precursor cells and enhances the functional properties of mature neutrophils. The mature human G-CSF (hG-CSF) is a glycoprotein mainly produced by activated monocytes and macrophages. In the present scenario, hG-CSF has become one of the most widely used hematopoietic growth factor because of its proven efficacy against different forms of neutropenia caused mainly due to chemotherapy, radiotherapy and organ transplants.
Amino Acid Sequence
Thr31-Pro204
MTPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQPPhysical Appearance:
Lyophilized powder
Buffer:
20mM Tris, 150mM NaCl, pH8.0
Reconstitution:
Reconstitute at 0.1-0.4 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution.
Note: Please avoid repeated freeze-thaw cycles