Human Collagen alpha-3(IV) chain (COL4A3)
Spodoptera frugiperda Sf9
cDNA coding for a minicollagen version of the human collagen IV α3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain). Expressed in insect cells with total 280 AA. Mw: 30.6 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
Liquid in 8M Urea.
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Store at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freezing/thawing cycles.
As an autoantigen, COL4A3 binds with IgG-type human auto-antibodies.