Gal-3，MAC-2, CBP 35, lectin L-29, AGER 3
Galectin-3 is expressed by a wide range of cell types including activated T cells, tumor cells, macrophages, osteoclasts, fibroblasts, and epithelial cells.
Galectin-3 has specific binding affinity for beta-galactoside sugar moieties and has functional roles during development, innate immunity, cell apoptosis, and tumor metastasis. Galectin-3 is associated with cancer, heart failure, stroke, and inflammation.
Expressed in E.coli with total 293AA. Mw: 31.1 kDa (calculated).
N-terminal 6xHis-tag, EK recognition site and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSENLY FQGAADNFSL HDALSGSGNPNPQGWPGAWG NQPAGAGGYPGASYPGAYPG QAPPGAYPGQ APPGAYPGAP GAYPGAPAPGVYPGPPSGPG AYPSSGQPSA TGAYPATGPYGAPAGPLIVPYNLPLPGGVV PRMLITILGTVKPNANRIAL DFQRGNDVAFHFNPRFNENN RRVIVCNTKL DNNWGREERQSVFPFESGKPFKIQVLVEPD HFKVAVNDAHLLQYNHRVKK LNEISKLGIS GDIDLTSASY TMI
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized at 1 mg/mL in PBS.
Standard ELISA test, Western Blot.
<0.01 EU per 1 μg of the protein by the LAL method.