Human Nuclear Pore Membrane Glycoprotein 210 (gp210)
The nuclear pore complex(NPC) is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. As a part of NPC, gp210 is detected with a prevalence of 26% in PBC and 4% in AIH.
Expressed in E.coli with total 238 AA. Mw: 24 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
Store at –20°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Immunodot analysis to determine functionality of protein.
Liquid in storage buffer (8M Urea, 10mM Tris, 50mM Na2HPO4, pH8.0).
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
As an autoantigen, gp210 binds with IgG-type human auto-antibodies.