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  • Human PM20D1 immunoassay kit

    SKU: 31700

     

    • INTRODUCTION

      PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.

    • ASSAY CHARACTERISTICS

      A. Sensitivity:

      The lowest level of human PM20D1 that can be measured by this assay is 0.209 ng/ml.

       

      B. Precision:

      Intra-assay Precision (Precision within an assay) < 3.7%.

      Inter-assay Precision (Precision between assays) < 3.8%.

      C. Spike

      Serum samples were assayed by adding 90 µl of

      sample and 10 µl of spike stock solution calculated

      to yield the intended 0, 1.25, 5 or 20 ng/ml spike

      concentration

       

       

      Spike level

      Expected

      (ng/ml)

      Observed

      (ng/ml)

      Recovery (%)

      Low spike (1.25 ng/ml)

      1.25

      1.22

      98

      Medium spike (5 ng/ml)

      5

             5.32

      106.5

      High spike (20 ng/ml)

      20

      18.65

      93.2

       

       

      D. Specificity: No cross reactivity with recombinant mouse PM20D1 protein

       

      E. Linearity: To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human PM20D1 were serially diluted with the 1×Assay buffer to produce samples with values within the dynamic range of the assay.

        Sample 1

       

       

       

       

      Dilution

      Measured (ng/ml)

      Expected (ng/ml)

      Recovery (%)

       

       

      1/2

      10.11

      9.51

      106.3

       

       

      1/4

      4.91

      4.9

      100.2

       

       

      1/8

      2.56

      2.69

      95.2

       

                       

       

      F. Sample : Serum and plasma

      G. Storage : 4°C

    • References:

      1. Korkmaz B et al., (2008) Biochimie 90:227-242.

      2.  Ginzberg HH et al., (2001) Am J Physiol Gastrointest Liver Physiol 281:G705-717.

    • DATA SHEET

    $450.00Price