Human Procalcitonin (PCT)
Origin: | Recombinant | Cat. No.: | 41670 |
Tag: | N-terminal 6xHis | Size: | 0.1 mg |
Source: | E.coli | Purity: | >90% |
Other names: | PCT | Species: | Human |
INTRODUCTION
Procalcitonin is a prohormone of calcitonin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock. Procalcitonin is also associated with systemic complications in humans suffering from acute pancreatitis.
DESCRIPTION
Expressed in E.coli with total 152 AA. Mw: 16.9 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 36 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolves completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Lyophilized at 1 mg/mL in PBS.
APPLICATION
Standard ELISA test, Western Blot.
ENDOTOXIN LEVEL
<0.2 EU/ug