P0 (Human Ribosomal Phosphoprotein P0)
  • P0 (Human Ribosomal Phosphoprotein P0)

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    Origin:

    Recombinant

    Cat. No.:

    41560

    Tag:

    N-terminal 6xHis

    Size:

    0.1 mg

    Source:

    E.coli

    Purity:

    >90%

    Other names:

    P0, RPLP0

    Species:

    Human

     
    • INTRODUCTION

      The ribosomal phosphoproteins (P protein antigens) are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 36 kDa, P1 (19 kDa) and P2 (17 kDa).

      Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients.

    • DATA SHEET

    • IMMUNOLOGICAL FUNCTION

      As an autoantigen, P0 binds with IgG-type human auto-antibodies.

    • AMINO ACID SEQUENCE

      MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQGAPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD

    • APPLICATIONS

      Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

    • FORMULATION

      Liquid in PBS(10mM Na2HPO4, 2mM KH2PO4, 2.7mM KCl,137mM NaCl, pH7.4).

    • RECONSTITUTION

      Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

       

    • DESCRIPTION

      Expressed in E.coli with total 360 AA.

      Mw: 39.2 kDa (calculated).

      N-terminal 6xHis-tag, Xpress Epitope, EK recognition site and TEV cleavage site, 44 extra AA (highlighted).

      Recombinant antigen for research use or manufacturing only.

    • STORAGE

      Store at –80°C.  Avoid repeated freezing/thawing cycles.

    • QUALITY CONTROL TEST

      BCA to determine quantity of the protein.

      SDS PAGE to determine purity of the protein.

    • SDS-PAGE Gel

      Refer to picture 

    $998.00Price
    Quantity

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