Human Ribosomal Phosphoprotein P0
The ribosomal phosphoproteins (P protein antigens) are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 36 kDa, P1 (19 kDa) and P2 (17 kDa).
Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients.
As an autoantigen, P0 binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Liquid at 0.58 mg/mL in PBS(10mM Na2HPO4, 2mM KH2PO4, 2.7mM KCl,137mM NaCl, pH7.4).
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Expressed in insect Sf9 cells with total 143 AA. Mw: 16.2 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Refer to picture