Human 2-oxoglutarate Dehydrogenase Complex E2 (OGDC-E2)
Origin:
Recombinant
Cat. No.:
41A072
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodopterafrugiperda Sf9
Purity:
>90%
Other names:
OGDC-E2, DLST
Species:
Human
INTRODUCTION
OGDC-E2 is the central E2 component of 2-oxoglutarate dehydrogenase complex (OGDC), which is a mitochondrial multienzyme complex involved in the maintenance of the cellular redox state.
Patients with primary biliary cirrhosis show autoantibodies against the E2 component of 2-oxoglutarate dehydrogenase complex.
DESCRIPTION
Expressed in insect Sf9 cells with a total of 411 AA.
Mw:44.5 KDa(calculated).
N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGADDLVTVKTPAFAESVTEGDVRWEKAVGDTVAEDEVVCEIETDKTSVQVPSPANGVIEALLVPDGGKVEGGTPLFTLRKTGAAPAKAKPAEAPAAAAPKAEPTAAAVPPPAAPIPTQMPPVPSPSQPPSGKPVSAVKPTVAPPLAEPGAGKGLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEIDMSNIQEMRARHKEAFLKKHNLKLGFMSAFVKASAFALQEQPVVNAVIDDTTKEVVYRDYIDISVAVATPRGLVVPVIRNVEAMNFADIERTITELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHGIFDRPVAIGGKVEVRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVLLLDL
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).
APPLICATION
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
IMMUNOLOGICAL FUNCTION
As an autoantigen, OGDC-E2 binds with IgG-type human auto-antibodies.