PDC-E2 (Human Pyruvate Dehydrogenase ComplexE2)
  • PDC-E2 (Human Pyruvate Dehydrogenase ComplexE2)

    SKU: 41A082

    ★ Download Datasheet PDF ★

     

     

    Origin:

    Recombinant

    Cat. No.:

    41A082

    Tag:

    N-terminal 6xHis

    Size:

    0.1 mg

    Source:

    Spodopterafrugiperda Sf9

    Purity:

    >90%

    Other names:

    PDC-E2

    Species:

    Human

    • INTRODUCTION

      The pyruvate dehydrogenase complex (PDC) is a mitochondrial multienzyme complex involved in the maintenance of the cellular redox state. PDC-E2 is the central E2 component of this complex.

      The anti-PDC-E2 antibodies belong to the group of Anti-Mitochondrial Antibodies (AMA-M2) and are strongly associated with PBC.

       

    • DATA SHEET

    • DESCRIPTION

      Expressed in insect Sf9 cells with total 586 AA.

      Mw: 62.72 KDa(calculated).

      N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted).

      Recombinant antigen for research use or manufacturing only.

    • AMINO ACID SEQUENCE

      MSYYHHHHHHDYDIPTTENLYFQGASLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL

    • STORAGE

      Store at –80°C.  Avoid repeated freezing/thawing cycles.

    • QUALITY CONTROL TEST

      BCA to determine quantity of the protein.

      SDS PAGE to determine purity of the protein.

    • FORMULATION

      Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).

    • APPLICATION

      Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels. 

       

    • SDS-PAGE Gel

      See image

    • IMMUNOLOGICAL FUNCTION

      As an autoantigen, PDC-E2 binds with IgG-type human auto-antibodies.

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