PDC-E2 (Human Pyruvate Dehydrogenase ComplexE2)

PDC-E2 (Human Pyruvate Dehydrogenase ComplexE2)

SKU: 41A082

★ Download Datasheet PDF ★

 

 

Origin:

Recombinant

Cat. No.:

41A082

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodopterafrugiperda Sf9

Purity:

>90%

Other names:

PDC-E2

Species:

Human

  • INTRODUCTION

    The pyruvate dehydrogenase complex (PDC) is a mitochondrial multienzyme complex involved in the maintenance of the cellular redox state. PDC-E2 is the central E2 component of this complex.

    The anti-PDC-E2 antibodies belong to the group of Anti-Mitochondrial Antibodies (AMA-M2) and are strongly associated with PBC.

     

  • DESCRIPTION

    Expressed in insect Sf9 cells with total 586 AA.

    Mw: 62.72 KDa(calculated).

    N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted).

    Recombinant antigen for research use or manufacturing only.

  • AMINO ACID SEQUENCE

    MSYYHHHHHHDYDIPTTENLYFQGASLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL

  • STORAGE

    Store at –80°C.  Avoid repeated freezing/thawing cycles.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • FORMULATION

    Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).

  • APPLICATION

    Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels. 

     

  • IMMUNOLOGICAL FUNCTION

    As an autoantigen, PDC-E2 binds with IgG-type human auto-antibodies.

$265.00Price

For bulk order (QTY: 5 or more), please contact us for quote

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