CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular regions. The cytoplasmic region with 148 aa contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. A secreted 310 aa residue variant of CDCP1 is also present by alternative splicing. It is also possible for the type I membrane CDCP1 to undergo proteolytic cleavage at its amino-terminal region, which is around 265 aa long. CDCP1 is present on the surface of epithelial and bone marrow-derived stem cells. The extracellular regions of mouse CDCP1 and human CDCP1 are 84% identical in terms of amino acids.

Antigen affinity purification

Recombinant human CDCP1 protein (Cat. No.: 41A323) expressed in mammalian cells.

The antibody detects human CDCP1 in western blot and Elisa.

Liquid in phosphate-buffered saline (PBS). Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Western blot – This antibody can be used as a primary antibody in western blot to detect human CDCP1.
ELISA - This antibody can be used as a capture antibody in human CDCP1 ELISA.