SARS-CoV-2 S1RBD IgG ELISA Kit [RUO]
SUPPLIED REAGENTS AND MATERIALS
- SARS-CoV-2 S1RBD coated ELISA plate:12 strips of 8 wells (96 wells in total) in a white strip holder and sealed in a foil bag with desiccant. Each well contains recombinant S1RBD of SARS-CoV-2. The microwell strips can be used separately. Place unused wells or strips in the provided plastic sealable storage bag together with the desiccant and return to 2-8°C. Once opened, stable for 4 weeks at 2-8°C.
- 5x Assay Buffer: 1 x 20 ml
- 10x Wash Buffer: 1 x 40 ml
- 100x Detection Antibody Solution: 1 x 0.12 ml
- Substrate Solution: 1 x 12 ml
- Stop Solution: 1 x 12 ml
- Blank Control: 1 x 1 ml
Positive Control (anti-S1RBD IgG): 1 vial
SARS-CoV-2 S1RBD IgG ELISA kit is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection and qualitative measurement of IgG class antibodies against the spike protein S1 receptor-binding domain (S1RBD) of SARS-CoV-2 virus in human blood.
This product is intended for use by professional persons only.
In December 2019, a novel coronavirus, now officially named as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been identified in Wuhan China, which caused the outbreak of a coronavirus-associated acute respiratory disease called coronavirus disease 19 (COVID-19). Signs and symptoms of COVID-19 may occur 2 to 14 days after infection, which include fever, cough, shortness of breath or difficulties in breathing, pain in the muscle and tiredness. In severe cases, the infection can further lead to pneumonia, severe acute respiratory syndrome (SARS), kidney failure and death.
The spike protein (S) is an envelope-anchored protein that mediates the recognition and binding of SARS-CoV-2 to host cells. S can be further cleaved by the host protease into two subunits called S1 and S2. S1 polypeptide contain a receptor binding domain (S1RBD) crucial for the specific recognition and interaction with the human receptor ACE2, which is the first and the most essential step for the virus infection.
ImmunoDiagnostics SARS-CoV-2 S1RBD IgG ELISA kit is a two-step incubation immunoassay kit. Recombinant spike protein S1 receptor-binding domain (S1RBD) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-S1RBD antibodies in human serum or plasma specimen. After a 1-hour incubation, anti-S1RBD antibodies are captured by immobilized S1RBD protein while the unbound components are washed away. Afterwards, a detection solution containing HRP-conjugated anti-human IgG is added for another 1-hour incubation, wherein HRP-conjugated anti-human IgG binds to the IgG class antibodies previously bound to S1RBD protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3′,5,5′-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti-S1RBP antibodies captured inside the wells.
STORAGE AND PREPARATION OF TEST SAMPLES
- Test samples are suggested to be assayed immediately after separation of serum or plasma, or preferably stored frozen (-200C or below) in aliquots. Multiple freeze-thaw cycles should be avoided. Duplicate test is recommended.
- Serum or plasma specimens with EDTA, sodium citrate or heparin can be tested. Highly lipaemic, icteric, or hemolytic specimens are not recommended. Specimens with visible microbial contamination should not be used.
- When required, vortex test serum or plasma samples at room temperature to ensure homogeneity. Then centrifuge samples at 10,000 to 15,000 rpm for 5 minutes prior to assay to remove particulates. Please do not omit this centrifugation step if samples are cloudy and containing particles.
STORAGE AND STABILITY
- The kit is stable until the expiry date only when stored at 2-8°C in sealed foil pouches. The expiry date is the last day of the month stated on the foil pouch and kit container.
- The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antigen-coated strips from the microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month. To assure maximum performance, protect the reagents from contamination with microorganism or chemicals during storage.
- Store the lyophilized positive control at -20°C for long-term storage. After reconstitution, store at 4°C for short-term storage (3 days) or store in aliquots at -80°C for long-term storage. Avoid frequent freeze-thaw cycles.
PUBLICATIONS CITING THIS PRODUCT
- Bavaro DF, Laghetti P, Milano E, Brindicci G, Volpe A, Lagioia A, Saracino A, Monno L. Anti‐spike S1 receptor‐binding domain antibodies against SARS‐CoV‐2 persist several months after infection regardless of disease severity. Journal of Medical Virology. 2021 Feb 16.