Recombinant Adiponectin with Flag Tag (Mouse)
Acrp30, GBP28, AdipoQ
Adiponectin is an adipokine exclusively expressed in adipose tissues with potent anti-diabetic, anti-atherogenic and anti-inflammatory functions.
In humans, decreased serum adiponectin levels are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased inflammation and increased risk for the development of type 2 diabetes, hypertension and coronary heart diseases.
Total 238 AA. Mw: 25.9 kDa (calculated). C-terminal flag-tag, 8 extra AA (highlighted).
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.
Avoid repeated freezing /thawing cycles.
Add sterile deionized water to prepare a working stock solution of approximately 1 mg/mL andlet the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized in 1 mg/mL in PBS.
Cell culture and/or animal studies, ELISA, Western blotting