Recombinant Human CDCP1
INTRODUCTION TO THE MOLECULE
CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. CDCP1 is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD).
The phosphorylation of CDCP1 is seen in many cancers, it is a putative stem cell marker, and is reported as a key regulator of EGF/EGFR-induced cell migration. It also interacts with cell-cell adhesion glycoproteins including CDH3/P-cadherin, CDH2/N-cadherin, membrane proteins including, SDC1/syndecan-1, SDC4/syndecan-4, and integral membrane serine protease ST14/MT-SP1. Its phosphorylation may also play a role in the regulation of anchorage versus migration and proliferation versus differentiation.
A DNA sequence encoding the human CDCP1 (Met1-Ser367) was expressed with a polyhistidine tag at the C-terminus (Uniprot No. Q9H5V8-3). Its predicted molecular mass of 39.1 kDa. The concentration of protein was determined by BCA.
Lyophilized from sterile PBS, pH 7.4.
For reconstitution, add 100 μl of deionized water, mix gently and incubate the reconstituted product for 10 minutes at room temperature prior to use.
Lyophilized protein can be stored at 2℃ to 8℃ for short-term, and at -20℃ to -80℃ for long term store. Reconstituted protein should be kept at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
QUALITY CONTROL TEST
BCA to determine concentration of the protein.
SDS PAGE to determine purity of the protein.