hFABP4 (Recombinant Human Fatty Acid Binding Protein4)
N-terminal 6xHis tag
FABP-4, also termed adipocyte fatty-acid binding protein (A-FABP) or AP2, has been shown to play a key role in obesity and metabolic syndrome. A-FABP knockout mice are substantially protected from insulin resistance, dyslipidemia, type 2 diabetes and fatty liver disease, despite of increased adipocity.
In clinical studies, increased circulating A-FABP levels are found in obese subjects and associated with non-alcoholic fatty liver disease, stroke, and carotid atherosclerosis.
Total 160 AA. Mw: 18 kDa (calculated). N-terminal His-tag and TEV cleavage site,28 extra AA (highlighted).
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.
Avoid repeated freezing /thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized in 1 mg/mL in PBS.
ELISA and Western blotting.