hFABP4 (Human Fatty Acid Binding Protein 4)
N-terminal 6xHis tag
Fatty-acid binding protein 4(FABP4), also termed adipocyte fatty-acid binding protein (A-FABP), or aP2, is a novel adipocyte-expressed factor which accounted for ~6% of total cellular proteins. Several animal experiments suggested that FABP-4 plays a key role in the link between obesity and various features of metabolic syndrome. Mice with targeted disruption of FABP-4 accompany FABP-5 almost completely protect against diet-induced obesity, insulin resistance, dyslipidemia, type 2 diabetes, and fatty liver disease. Studies in human found FABP-4 serum levels were significantly increased in overweight and obese subjects, which predicted the risk to develop metabolic syndrome and type 2 diabetes. Additionally, serum FABP-4 levels were associated with carotid atherosclerosis and coronary artery disease.
Total 160 AA. Mw: 18 kDa (calculated).
N-terminal His-tag and TEV cleavage site, 28 extra AA (highlighted).
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.
Avoid repeated freezing /thawing cycles.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilized in 1 mg/mL in PBS.
ELISA and Western blotting.