Human Fatty Acid Binding Protein 4 (FABP4)
Type: | Recombinant | Cat. No.: | 41030 |
Tag: | N-terminal 6xHis tag | Size: | 0.1 mg |
Source: | E.Coli | Purity: | >95% |
Other names: | aP2; A-FABP; | Species: | Human |
INTRODUCTION
Fatty acid binding protein 4(FABP4), also termed adipocyte fatty acid binding protein (A-FABP), or aP2, is a novel adipocyte-expressed factor which accounted for ~6% of total cellular proteins. Several animal experiments suggested that FABP-4 plays a key role in the link between obesity and various features of metabolic syndrome. Mice with targeted disruption of FABP-4 accompany FABP-5 almost completely protect against diet-induced obesity, insulin resistance, dyslipidemia, type 2 diabetes, and fatty liver disease. Studies in human found FABP-4 serum levels were significantly increased in overweight and obese subjects, which predicted the risk to develop metabolic syndrome and type 2 diabetes. Additionally, serum FABP-4 levels were associated with carotid atherosclerosis and coronary artery disease.
DESCRIPTION
Total 160 AA. Mw: 18 kDa (calculated).
N-terminal His-tag and TEV cleavage site, 28 extra AA (highlighted).
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAMGSMCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA
ENDOTOXIN LEVEL
<0.2 EU/ug.
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.
Avoid repeated freezing /thawing cycles.
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Lyophilized in 1 mg/mL in PBS.
APPLICATION
ELISA and Western blotting.