hFABP4 (Human Fatty Acid Binding Protein 4)

hFABP4 (Human Fatty Acid Binding Protein 4)

SKU: 41030

★ Download Datasheet PDF ★

 

 

Type:

Recombinant

Cat. No.:

41030

Tag:

N-terminal 6xHis tag

Size:

0.1 mg

Source:

E.Coli

Purity:

>95%

Other names:

aP2; A-FABP;

Species:

Human

  • INTRODUCTION

    Fatty-acid binding protein 4(FABP4), also termed adipocyte fatty-acid binding protein (A-FABP), or aP2, is a novel adipocyte-expressed factor which accounted for ~6% of total cellular proteins. Several animal experiments suggested that FABP-4 plays a key role in the link between obesity and various features of metabolic syndrome. Mice with targeted disruption of FABP-4 accompany FABP-5 almost completely protect against diet-induced obesity, insulin resistance, dyslipidemia, type 2 diabetes, and fatty liver disease. Studies in human found FABP-4 serum levels were significantly increased in overweight and obese subjects, which predicted the risk to develop metabolic syndrome and type 2 diabetes. Additionally, serum FABP-4 levels were associated with carotid atherosclerosis and coronary artery disease.

  • DATA SHEET

  • DESCRIPTION

    Total 160 AA. Mw: 18 kDa (calculated).

    N-terminal His-tag and TEV cleavage site, 28 extra AA (highlighted).

  • AMINO ACID SEQUENCE

    MSYYHHHHHHDYDIPTTENLYFQGAMGSMCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA

  • ENDOTOXIN LEVEL

    <0.2 EU/ug.

  • STORAGE

    Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.

    Avoid repeated freezing /thawing cycles.

  • RECONSTITUTION

    Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • FORMULATION

    Lyophilized in 1 mg/mL in PBS.

  • APPLICATION

    ELISA and Western blotting.

     

  • SDS-PAGE Gel

    See pictures.

$200.00Price

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