Recombinant Human Tumor necrosis factor alpha, soluble form (TNF-α)
His tag on N terminal
TNF-α is a cytokine produced by monocytes and macrophages. It consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain. Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 KDa soluble trimer. TNF-α functions as a modulator of immune response and plays a role in the pathogenesis of many disease states.
AMINO ACID SEQUENCE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C for long term storage.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Lyophilised in 1 mg/mL in PBS.
ELISA and Western blotting.
As an autoantigen, OGDC-E2 binds with IgG-type human auto-antibodies.