SARS-CoV-2 Nucleocapsid Protein (NP)
Origin:
Recombinant
Cat No.:
41A220
Source:
E.coli
Size:
0.1 mg
Tag:
No Tag
Purity:
>95%
Antigenicity Test
Antigenicity validated in 16 patient serum samples via ELISA by coating SARS-CoV-2 NP as capture antigen, with a detection rate of 93.75%.
Strong antiginetic response even in 70000-fold diluted patient serum.
Amino Acid Sequence
SDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
INTRODUCTION
Since December 2019, outbreak of SARS-CoV-2 infection has become a major epidemic threat in China and brought back the attention of pathogenic coronavirus to the spotlight. Nucleocapsid protein (NP) is the most abundant protein on the helical nucleocapsid of coronaviruses, which envelopes the entire genomic RNA. NP also interacts with other viron structural proteins to play important roles during host cell entry and virus particle assembly and release.
Expressed in E.coli with total 418 AA. Mw: 45.5 KDa (calculated).
Recombinant antigen for research use or manufacturing only.
DATA SHEET
AMINO ACID SEQUENCE
SDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Antigenicity Test
Antigenicity validated in 16 patient serum samples via ELISA by coating COVID-19 NP as capture antigen, with a detection rate of 93.75%.
Strong antiginetic response even in 70000-fold diluted patient serum.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
As liquid with vials containing NP to 1.0mg/mL in 50mM Tris, 300mM NaCl, 10% Glycerol, PH8.0.
IMMUNOLOGICAL FUNCTION
As an autoantigen, NP binds with IgG-type human auto-antibodies.
PUBLICATIONS CITING THIS PRODUCT
- Zhou R, To KK, Wong YC, Liu L, Zhou B, Li X, Huang H, Mo Y, Luk TY, Lau TT, Yeung P. Acute SARS-CoV-2 infection impairs dendritic cell and T cell responses. Immunity. 2020 Oct 13;53(4):864-77.