SARS-CoV-2 Open Reading Frame-8 (ORF8), Truncated
< 5 EU/mg
Expressed in E. coli with total 96AA. MW: 17.1 kDa.
Truncated ORF8 (27aa-122aa) (excluding signal peptide and a-helix region).
Includes rTEV cleavage site (ENLYFQG).
The genome of SARS-CoV-2 encodes accessory genes that differ significantly within coronaviruses and contribute to the virus pathogenicity. Among accessory genes, open reading frame 8 (ORF8) stands out by being highly variable and showing structural changes suspected to be related with the virus ability to spread. SARS-CoV-2 ORF8 is a 122 amino acid protein with less than 20% sequence identity to SARS-CoV ORF8. It has an N-terminal signal sequence for the protein secretion, followed by an alpha-helix domain and a beta-sheet containing six strands. The recombinant ORF8 generated by Immunodiagnostics does not contain the a-helix region to increase the solubility.
AMINO ACID SEQUENCE
Liquid containing ORF8 (2.0 mg/ml) in 50mM Tris, 300mM NaCl, 10% Glycerol, pH8.0.
Store protein at –20°C or –80°C. Avoid repeated freezing / thawing cycles.
ELISA and Western blotting.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.